Biopolym. Cell. 1989; 5(4):71-75.
http://dx.doi.org/10.7124/bc.0000D7
Cell Biology
Uptake of oligonucleotide alkylating derivatives by the cells in the presence of substances stimulating transfection
1Boutorin A. S., 1Vlassov V. V., 1Ivanova E. M., 1Ryte A. S., 1Yurchenko L. V., 1Shishkina I. G., 1Yakubov L. A.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR

Abstract

The effect of components facilitating transfection on the uptake of the alkylating 4-(N-2-chloroethyl-N-methylamino) -benzyl 5'-[32P]phosphamides of oligodeoxyribonucleotides of various length and structure by the L-929 mouse fibroblasts and Krebs-2 ascite carcinoma cells was investigated. Dimethylsulfoxide, DEAE-dextran and polylysine had a little or no effect on the uptake. Substantial effect has been observed when oligonucleotide derivatives were coprecipitated with the cells by calcium chloride from the phosphate buffer. Uptake of the derivatives by cells increased at least by one order of magnitude and the level of nucleic acids specific modification within the cells —by factor of 6.
Full text: (PDF, in Russian)

References

[1] Knorre DG, Vlassov VV. Complementary-addressed (sequence-specific) modification of nucleic acids. Prog Nucleic Acid Res Mol Biol. 1985;32:291-320.
[2] Zamecnik PC, Stephenson ML. Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide. Proc Natl Acad Sci U S A. 1978;75(1):280-4.
[3] Stephenson ML, Zamecnik PC. Inhibition of Rous sarcoma viral RNA translation by a specific oligodeoxyribonucleotide. Proc Natl Acad Sci U S A. 1978;75(1):285-8.
[4] Vlasov VV, Godovikov AA, Zarytova VF, Ivanova EM, Knorre DG. Suppression of immunoglobulin synthesis in myeloma MORS 21 cells by an alkylating oligonucleotide derivative complementary to the mRNA coding for light-chain immunoglobulin. Dokl Akad Nauk SSSR. 1984;276(5):1263-5.
[5] Cazenave C, Loreau N, Toulme JJ, Helene C. Anti-messenger oligodeoxynucleotides: specific inhibition of rabbit beta-globin synthesis in wheat germ extracts and Xenopus oocytes. Biochimie. 1986;68(9):1063-9.
[6] Ivanova EM, Karpova GG, Knorre DG, Popova VS, Rait AS. Selective modification of polyadenyl fragments of mRNA from Krebs-2 ascites carcinoma cells by an alkylating derivative of nonathymidilyluridine. Mol Biol (Mosk). 1984;18(3):613-9.
[7] Juliano R, Mayhew E. Interaction of polynucleotides with cultured mammalian cells. 1. Uptake of RNA by Ehrlich ascites carcinoma cells. Exp Cell Res. 1972;73(1):3-12.
[8] Warden D, Thorne HV. The infectivity of polyoma virus DNA for mouse embryo cells in the presence of diethylaminoethyl-dextran. J Gen Virol. 1968;3(3):371-7.
[9] Graham FL, van der Eb AJ. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973;52(2):456-67.
[10] Stenberg K, Oberg B, Chattopadhyaya JB. Precipitation of nucleotides by calcium phosphate. Biochim Biophys Acta. 1982;697(2):170-3.
[11] Zarytova VF, Ivanova EM, Romanenko VP. Synthesis of oligonucleotides by PhosPhotriester aPProach in chloroform. Russian Journal of Bioorganic Chemistry. 1983; 9(4):516-21.
[12] Bausk EV, Gorn VV, Lebedev AV. General scheme of the solid-Phase PhosPhotriester synthesis of 5'-PhosPhorylated-oligodeoxyribonucleotides on the basis of β-cyanoethyl P-chloroPhenyl esters of N-acylnucleoside-5'-PhosPhates. Russian Journal of Bioorganic Chemistry. 1985; 11(6):815-20.
[13] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold SPring Harbor, 1982; 545 P.
[14] Godovikova TS, Zarytova VF, Khalimskaya LM. Reactive PhosPhamidates of mono- and dinucleotides. Russian Journal of Bioorganic Chemistry. 1986; 12(4):475-81.
[15] Vlassov VV, Gorokhova OE, Ivanova EM, Kutyavin IV, Yurchenko LV, Yakubov LA, Abdukayumov MN, Skoblov YuS. Interaction of alkylating oligonucleotide derivatives with mouse fibroblasts. Biopolym Cell. 1986; 2(6):323-7.