Deoxyriboanalog of the anticodon arm of yeast tRNAPhe is not able to codon-dependent binding to small ribosomal subunits of E. coli and the rabbit liver

Soldatkin, K. A.; Kovalchuk, O. V.; Potapov, A. P.; Elskaya, A. V.; Krynetskaya, N. F.; Dolinnaya, N. G.; Shabarova, Z. A.

doi:10.7124/bc.0000D5

Biopolym. Cell. 1989; 5(4):62-66.

http://dx.doi.org/10.7124/bc.0000D5

Structure and Function of Biopolymers

Deoxyriboanalog of the anticodon arm of yeast tRNA^Phe is not able to codon-dependent binding to small ribosomal subunits of E. coli and the rabbit liver

1Soldatkin K. A., 1Kovalchuk O. V., 1Potapov A. P., 1Elskaya A. V., 1Krynetskaya N. F., 1Dolinnaya N. G., 1Shabarova Z. A.

Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR

Abstract

Oligonucleotide d(CCAGACTGAAGATCTGG) has been used to study influence of sugar-phosphate backbone modifications on the interaction of tRNA anticodon region with ribosomes. Its sequence corresponds to nonmodified tRNA^Phe_yeast anticodon arm. The oligonucleotide is shown to form an intramolecular «loop», but nevertheless it is not able to be bound to 305 and 40S ribosomes of E. coli and rabbit liver in the presence of ribo-(poly(U)) or deoxyribo-(poly(dT)) messenger. The addition of neomycin B promotes no changes in the situation.

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