Biopolym. Cell. 1989; 5(3):88-94.
http://dx.doi.org/10.7124/bc.0000C8
Structure and Function of Biopolymers
5'-Phosphorylation of natural nucleosides and their modified analogs by intact Erwinia herbicola cells: specificity with respect to donors and acceptors of phosphate group
1Zinchenko A. I., 1Popov I. L., 1Barai V. N., 2Mikhailopulo I. A.
  1. Institute of Microbiology, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR
  2. Istitute of Bioorganic Chemistry, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR

Abstract

Nucleoside-phosphotransferase reaction catalyzed by intact cells of Erwinia herbicola 47/3 has been studied. p-Nitrophenyl phosphate is found to manifest the highest activity among phosphate donors tested. All the natural ribonucleotides and ribose-5-phosphate proved to be highly efficient as well. The activity of ribonucleosides as acceptors of phosphate group decreases in the following order: guanosine>uridine>ribothymidine>cytidine>adenosine>inosine>xanthosine. 2'-Deoxynucleosides proved to be less efficient phosphate acceptors as compared to the corresponding ribonucleosides. The findings obtained suggest that intact cells of Erw. herbicola 47/3 may be used to produce modified nucleoside-5'-monophosphates in preparative amounts
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