Biopolym. Cell. 1989; 5(1):72-77.
Gene-Engineering Biotechnology
Restriction enzyme analysis and localization of Bacillus subtilis chromosomal fragment partially suppressing recB recC mutations of Escherichia coli
- V. I. Ulyanov-Lenin State University of Kazan,
Kazan, USSR
Abstract
The original plasmid pKUl partially suppressing recB21 recC22 mutations of E. coli contains a 6.9 kb chromosomal fragment of B. subtilis. Using this plasmid an inte-grative vector for B. subtilis was constructed. The complementation analysis revealed that the cloned fragment contained a new B. subtilis gene, which codes the exonuclease activity of ATP-dependent DNAse and differs from recE5 and recH genes. The new gene, designated as recQ, has been located in glyB region on 75В° of the B. subtilis genetic map.
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