Biopolym. Cell. 2025; 41(3):222.
Bioorganic Chemistry
Spectral-fluorescent response of dioxaborine dyes with varying chromophore charge in the presence of serum albumins
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143 - Taras Shevchenko National University of Kyiv
64, Volodymyrska Str., Kyiv, Ukraine, 01601 - Institute of organic chemistry of the NAS of Ukraine
5, Kukharya Str., Kyiv, Ukraine, 02094
Abstract
Aim. The aim of the work is the study of a spectral-fluorescent response of a series of dioxaborine dyes in the presence of serum albumins. Methods. UV-Vis absorption and fluorescence spectroscopy. Results. The fluorescent response of eight polymethine dyes (six of them being dioxaborine-based) in the presence of human, bovine, and equine serum albumins was studied and compared to the response in the presence of DNA and ovalbumin. It was shown that dianionic dioxaborine A74 and thiobarbituric-based merocyanine In4tb demonstrated0 the most promising properties as fluorescent probes for human serum albumin detection, with fluorescence intensity increasing by 62- and 56-fold, respectively. Meanwhile, A74 shows the highest selectivity to HSA among other serum albumins: in the presence of ESA and BSA, the signal increased by only 28-fold and 7.9-fold, respectively. Thus, one can suppose that A74 may be sensitive to slight variations in the globular conformation of serum albumins. Besides, dioxaborine merocyanines B33 and B36 demonstrated nearly 10-fold fluorescence intensity increase in the presence of serum albumins. Conclusions. The most promising dyes A74 and In4tb can be further studied for various applications that require fluorescent detection or imaging of HSA, and, in the case of A74, as a probe sensitive to minor changes in the HSA globular structure.
Keywords: dioxaborines, polymethine dyes, fluorescent probes, serum albumins
Full text: (PDF, in English)
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