Aim. Non-small cell lung cancer (NSCLC) is characterized by a wide range of genetic abnormalities that impact the disease prognosis. However, the data on frequency of various genetic disorders in lung cancer in the Ukrainian population are limited. Our study aimed to evaluate the frequency of genetic alterations in NSCLC samples to identify potential candidates for personalized treatment. Conclusions. The high frequency and variety of genetic alterations in NSCLC determine the expediency of NGS testing to personalize the treatment of patients with NSCLC.