Biopolym. Cell. 1988; 4(6):310-313.
Cell Biology
Dynamics and mechanism of the antibody thermoinactivation
1Bobrovnik S. A., 1Starodub N. F.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


It is shown that the kinetic plots of the antibody thermoinactivation in semilogarithmic scale are approximated by two straight lines intersecting at a certain angle. The model of the antibody thermoinactivation process is proposed. Both the kinetic characteristics of the process studied at various temperatures and its thermodynamic parameters are calculated.


[1] Bobrovnik S. A. Enumeration of specific antibody-forming cells by the immunofluorescent imprint technique. EOS J. Immunol, and Immunopharmacol. 1986; 15(3):178-182.
[2] Gripenberg M, Kurki P. Demonstration of human autoantibodies by quantitative enzyme immunoassays. J Immunol Methods. 1986;92(2):145-59.
[3] Mukkur TK. Thermodynamics of hapten-antibody interactions. CRC Crit Rev Biochem. 1984;16(2):133-67.
[4] Bobrovnik SA. Determination of rate constants for certain biexponential biological processes. Biopolym. Cell. 1987; 3(5):245-50.
[5] Dixon M, Webb EC. Enzymes. New York, Acad. Press, 1964; 950 p.