Biopolym. Cell. 2021; 37(4):315-322.
http://dx.doi.org/10.7124/bc.000A5D
Viruses and Cell
Complete Genome Sequence of Serratia Phage 4S Isolated from Wastewater in Ukraine
1Dukhno E. A., 1Kornienko N. O., 1Shybanov S. R., 1Budzanivska I. G., 1Kharina A. V.
  1. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601

Abstract

Aim. To isolate and characterize phage of Serratia marcescens bacteria. Methods. Phylogenetic analysis. Results. The complete genome of Serratia phage 4S represents a 173,061-bp double-stranded DNA (dsDNA) with a GC content of 39.9 %. The Basic Local Alignment Search Tool (BLAST) results indicated that the closest relative to Serratia phage 4S is Serratia phage CBH8 (7 % query coverage, 76 % identity). According to the electron micrograph images Serratia phage 4S belongs to the order Caudovirales and the family Myoviridae. Following a phylogenetic analysis of Serratia phage 4S MCP (Major Capsid Protein), our results showed that its MCP was highly homologous to Acinetobacter and Enterobacter phages and on the contrary distant from the MCP of Klebsiella phages. The phylogenetic analysis of Serratia phage 4S DNA helicase indicated that it was highly homologous to Yersinia and Enterobacter phages, and on the contrary distant from DNA helicase of Klebsiella phages. Conclusions. Serratia phage 4S has a lytic pathway, which means that it can be considered for further investigation as a control agent against bacterial infections caused by Serratia marcescens.
Keywords: Serratia marcescens, Serratia phage, sequencing, phylogenetic analysis
Full text: (PDF, in English)

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