Biopolym. Cell. 2021; 37(4):270-277.
http://dx.doi.org/10.7124/bc.000A59
Molecular Biomedicine
MHRT expression during remote ischemic preconditioning in patients with coronary artery disease
1Khetsuriani M., 2Ioffe N. O., 1Drevytska T. I., 3Niekrasova V. O., 1Dosenko V. E.
  1. O. O. Bogomoletz Institute of Physiology, NAS of Ukraine
    4, Akademika Bogomoltsa Str., Kyiv, Ukraine, 01004
  2. Amosov National Institute of Cardiovascular Surgery, National Academy of Medical Sciences of Ukraine
    6, Mykoly Amosova Str., Kyiv, Ukraine, 03038
  3. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601

Abstract

Aim. To access the effect of remote ischemic preconditioning (RIPC) on hemodynamic parameters and expression of lncRNA MHRT in patients with coronary artery disease during isolated coronary artery bypass graft surgery. Methods. The hemodynamic parameters of 29 patients with sham and RIPC procedures were analyzed. RIPC was modulated by three cycles of blocking blood flow in the arm for 5 minutes followed by restoring it for another 5 minutes. Expression of MHRT was measured in myocardial tissue and plasma by real-time polymerase chain reaction. Results. Hemodynamic parameters of patients in the RIPC-group had higher (p < 0.05) values of cardiac index, systolic blood pressure, diastolic blood pressure, stroke volume index compared to the sham group. Systemic vascular resistance index decreased in RIPC-group. The expression level of MHRT in the myocardium was 2.5 times lower than in the sham group (p<0.05). MHRT expression in plasma fall[s] significantly, by more 15 times, (p < 0.0001) in the RIPC-group. Conclusions. A decrease in MHRT expression can be a consequence of the protective effect of RIPC and can be used as a cardiac biomarker.
Keywords: long noncoding RNA, ischemic preconditioning, MHRT, coronary artery disease, coronary artery bypass grafting
Full text: (PDF, in English)

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