Biopolym. Cell. 2019; 35(6):476-485.
http://dx.doi.org/10.7124/bc.000A1A
Viruses and Cell
Genetic diversity of Plum pox virus in Ukraine
1Kutsenko O. V., 1Budzanivska I. G., 1Shevchenko O. V.
  1. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601

Abstract

Aim. Establishing the genetic diversity of Plum pox virus (PPV) in Ukraine. Methods. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), polymerase chain reaction with reverse transcription (RT-PCR), DNA sequencing and phylogenetic analysis. Results. The samples with visual symptoms of virus infection were collected from different regions of Ukraine and diagnosed with DAS-ELISA. Subsequently, the amplicons of the coat protein (CP) gene were obtained using RT-PCR and sequenced. Obtained viral cDNAs were analyzed using phylogenetic methods. As a result, the genetic diversity of PPV in different regions of Ukraine has been elucidated. The phylogenetic relationships between Ukrainian isolates were studied with subsequent comparison to PPV isolates from neighboring countries. Conclusions. The PPV strains M and D circulate in Ukraine. For the first time, PPV was found in the Kharkiv region. The PPV isolates circulating in Ukraine are most similar to the isolates from Germany, Turkey, Hungary, and Canada. The results can be used in the future to predict the spread of the virus in different regions of Ukraine and neighboring countries, to establish its origin and predict the development of possible epidemics.
Keywords: Plum pox virus, coat protein gene, phylogenetic analysis, sequencing, genetic diversity, Ukraine
Full text: (PDF, in English)

References

[1] Atanassov D. Plum pox. A new virus disease. Ann Univ Sofia Fac Agric Silvic 1932; (11):49-69.
[2] Ratushnyak LK. Diagnostics of plums in the stone gardens. Quarantine and plant protection. 2002; (48):199-207.
[3] García JA, Glasa M, Cambra M, Candresse T. Plum pox virus and sharka: a model potyvirus and a major disease. Mol Plant Pathol. 2014;15(3):226-41.
[4] Subr Z, Glasa M. Unfolding the secrets of plum pox virus: from epidemiology to genomics. Acta Virol. 2013;57(2):217-28.
[5] Maejima K, Himeno M, Komatsu K, Takinami Y, Hashimoto M, Takahashi S, Yamaji Y, Oshima K, Namba S. Molecular epidemiology of Plum pox virus in Japan. Phytopathology. 2011;101(5):567-74.
[6] Cirilli M, Geuna F, Babini AR, Bozhkova V, Catalano L, Cavagna B, Dallot S, Decroocq V, Dondini L, Foschi S, Ilardi V, Liverani A, Mezzetti B, Minafra A, Pancaldi M, Pandolfini T, Pascal T, Savino VN, Scorza R, Verde I, Bassi D. Fighting Sharka in Peach: Current Limitations and Future Perspectives. Front Plant Sci. 2016;7:1290.
[7] Kondratenko P, Udovichenko V. Plum pox virus (PPV) in Ukraine. Bulletin OEPP EPPO Bulletin. 2006; 36(2):217.
[8] Chirkov S, Ivanov P, Sheveleva A, Kudryavtseva A, Prikhodko Y, Mitrofanova I. Occurrence and characterization of plum pox virus strain D isolates from European Russia and Crimea. Arch Virol. 2016;161(2):425-30.
[9] James D, Thompson D. Hosts and symptoms of Plum pox virus: ornamental and wild Prunus species. EPPO Bulletin. 2006; 36(2):222-224.
[10] García JA, Glasa M, Cambra M, Candresse T. Plum pox virus and sharka: a model potyvirus and a major disease. Mol Plant Pathol. 2014;15(3):226-41.
[11] Mavrodieva V, James D, Williams K, Negi S, Varga A, Mock R, Levy L. Molecular Analysis of a Plum pox virus W Isolate in Plum Germplasm Hand Carried into the USA from the Ukraine Shows a Close Relationship to a Latvian Isolate. Plant Dis. 2013;97(1):44-52.
[12] Prikhodko Yu N, Mazurin E S, Zhivaeva T S, Schneider Yu A, ,Sokolova E E. The study of plum pox virus strains. Plant protection and quarantine. 2011; (11). 29-32.
[13] Norkus T, Staniulis J, Žižyte M, Melnyk M, Yusko L, Snihur H, Budzavinska I, Polischuk V. Molecular identifica-tion of Plum pox virus isolates from Lithuania and Ukraine. Zemdirbyste-Agriculture. 2008; 95(3):277-85.
[14] Kyrychenko A, Shcherbatenko I, Antipov I, Hrynchuk K. Typing of Plum pox virus isolates in the central Ukraine. Microbiol Z. 2017; 79(3):115-24.
[15] Budzanivska I, Usko L, Gospodaryk A, Melnyk M, Polischuk V. Epidemiology of sharka disease in Ukraine. Acta Hort. 2011; 899:57-63.
[16] Cambra M, Boscia D, Myrta A, Palcovics L, Navrátil M, Barba M, Gorris MT, Capote N. Detection and characterization of Plum pox virus: serological methods. Bulletin OEPP EPPO Bulletin. 2006; 36(2). 254-261.
[17] Clark MF, Adams AN. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol. 1977;34(3):475-83.
[18] Sochor J, Babula P, Adam V, Krska B, Kizek R. Sharka: the past, the present and the future. Viruses. 2012;4(11):2853-901.
[19] Wetzel T, Candresse T, Ravelonandro M, Dunez J. A polymerase chain reaction assay adapted to plum pox potyvirus detection. J Virol Methods. 1991;33(3):355-65.
[20] Olmos A, Cambra M, Dasi MA, Candresse T, Esteban O, Gorris MT, Asensio M. Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA. J Virol Methods. 1997;68(2):127-37.
[21] Subr Z, Pittnerová S, Glasa M. A simplified RT-PCR-based detection of recombinant Plum pox virus isolates. Acta Virol. 2004;48(3):173-6.
[22] Nemchinov L, Hadidi A. Specific oligonucleotide primers for the direct detection of plum pox potyvirus-cherry subgroup. J Virol Methods. 1998; 70(2):231-4.