Biopolym. Cell. 2018; 34(1):49-58.
http://dx.doi.org/10.7124/bc.000970
Viruses and Cell
Effect of fluorinated N-alkylthioamides on HSV-1 multiplicity
1Biliavska L. O., 1Pankivska Y. B., 1Povnitsa O. Yu., 2Pikun N. V., 2Shermolovich Y. G., 1Zagorodnya S. D
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680
  2. Institute of Organic Chemistry, NAS of Ukraine
    5, Murmanska Str., Kyiv, Ukraine, 02660

Abstract

The number of promising viral targets and classes of compounds with substantial antiherpetic properties considerably increased during the last decade. However, no new effective and low-toxicity clinical drugs against both wild-type viruses and drug-resistant strains have appeared. This situation makes the search for new antiherpetic drugs and their new targets a high priority. Aim. To study the effect of fluorinated N-alkylthioamides on the herpes simplex virus-1 (HSV-1) multiplicity. Methods. The influence of these compounds on the multiplicity and infectivity of HSV-1 was determined by the MTT-assay, virucidal assay, adsorption and penetration assays, PCR and infectious virus yield reduction assay. Results. The 10S-23 and 10S-24 compounds pre-vented the adsorption and penetration of HSV-1 into cells up to 26%. HSV-1 DNA replication was moderately inhibited by compound 10S-24 (to 39 %). It was found that the compounds 10S-23 and 10S-24 in concentration of 100 – 33 μg/ml reduce the titer of virus obtained de novo by 70 – 99% and >99%, respectively. Conclusion. These results suggest that the 10S-24 compound may be used as a therapeutic agent to reduce the penetration, replication and translation of HSV-1.
Keywords: Herpes simplex virus, Fluorinated N-alkylthioamides, Antiviral activity
Full text: (PDF, in English)

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