Molecular characterization and phylogenetic analysis of Ukrainian isolates of Cucumber mosaic virus based on the partial sequences of three genes

Shevchenko, T. P.; Tymchyshyn, O. V.; Kosenko, I. A.; Budzanivska, I. G.; Shevchenko, O. V.; Polishchuk, V. P.

doi:10.7124/bc.00096E

Biopolym. Cell. 2018; 34(1):32-40.

http://dx.doi.org/10.7124/bc.00096E

Viruses and Cell

Molecular characterization and phylogenetic analysis of Ukrainian isolates of Cucumber mosaic virus based on the partial sequences of three genes

1Shevchenko T. P., 1Tymchyshyn O. V., 1Kosenko I. A., 1Budzanivska I. G., 1Shevchenko O. V., 1Polishchuk V. P.

Educational and Scientific Center "Institute of Biology and Medicine",
Taras Shevchenko National University of Kyiv
64/13, Volodymyrska Str., Kyiv, Ukraine, 01601

Abstract

Aim. In the current work, we carried out the phylogenetic analysis of the Ukrainian isolates of Cucumber mosaic virus using incomplete sequences of several viral genes. Methods. ELISA, RT-PCR, DNA sequencing and phylogenetic analysis. Results. The symptomatic samples of plants from different regions of Ukraine were collected and tested for CMV infection. Partial sequences of the genes encoding coat protein, movement protein and 2b protein of four Ukrainian CMV isolates were amplified and analyzed. These isolates were shown to belong to subgroups IA and IB. The coat protein, movement protein and 2b protein gene sequences demonstrated high intragroup homology. For isolates CMV-Ukr-28 and CMV-Ukr-58, the unique amino acid substitutions were detected in 2b protein sequences which were considered independent of the host plant. Conclusions. The isolates of Cucumber mosaic virus which circulate in Ukraine and are described in this study, belong to the most widespread phylogenetic subgroups IA and IB which are also found in other European countries. The obtained data may indicate separate routes of CMV infection, as well as the ongoing virus microevolution in Ukraine.

Keywords: Cucumber mosaic virus, coat protein gene, , , phylogenetic analysis

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