Biopolym. Cell. 2016; 32(5):377-380.
Viruses and Cell
Phylogenetic analysis of influenza A viruses (H1N1)pdm circulating during 2014–2015 epidemic season in Ukraine
1Radchenko L. V., 1, 2Smutko O. Yu., 1Fesenko A. Yu., 1Mironenko A. P.
  1. Gromashevsky L. V. Institute of Epidemiology and Infection Diseases, NAMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038
  2. Educational and Scientific Center "Institute of Biology",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601


Aim. To analyze the pandemic influenza A(H1N1) strains that circulated in Ukraine during 2014–2015 epidemic season. Methods. Real-time polymerase chain reaction (RT-PCR). The phylogenetic trees were constructed using MEGA 6 software. Results. The strains, that were circulating in Ukraine were similar to those of the A/California/07/2009 (H1N1) vaccine strain. Most strains belonged to the clade 6B. The H275Y mutation in the neuraminidase (NA) gene was identified, that confers resistance to oseltamivir. Conclusions. Based on the nucleotide sequences of HA and NA genes, we constructed the influenza virus phylogenetic trees. The influenza virus isolated in 2014–2015 epidemic season had a specific mutation, associated with resistance to antiviral drugs such as oseltamivir.
Keywords: A(H1N1)pdm influenza viruses, mutation, phylogenetic analysis, isolate


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