Biopolym. Cell. 2015; 31(4):285-293.
http://dx.doi.org/10.7124/bc.0008ED
Molecular and Cell Biotechnologies
Construction and analysis of the transgenic carrot and celery plants expressing the recombinant thaumatin II protein
1Luchakivska Yu. S., 1Komarnytskii I. K., 2Kurchenko I. M., 2Yurieva O. M., 2Zhytkevich N. V., 1Kuchuk M. V.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim To obtain the transgenic carrot and celery plants able to express recombinant thaumatin II in order to increase plant stress tolerance. Methods. Agrobacterium-mediated transformation of the carrot and celery seedlings was used for obtaining the transgenic plants. Presence and transcription of the transgene in plant tissues were proved by PCR and RT-PCR analysis. The plants were tested for the biotic stress tolerance by in vitro antifungal and antibacterial activity assays and for the salinity and osmotic stress tolerance by plant survival test in presence of NaCl and PEG in different concentrations. Results. Transgenic plants able to express recombinant thaumatin II gene (transcription proved for 60–100 %) were obtained by agrobacterial transformation. The transgenic carrot plant extracts inhibited the growth of the studied phytopathogenic bacteria strains but exhibited no antifungal activity. Survival level of transgenic plants under the salinity and osmotic stress effect was definitely higher comparing to the untransgenic ones. The analysis of the photosynthetic pigment content in the transgenic carrot plants showed no significant difference of this parameter under salinity stress that may indicate a possible protective activity of the recombinant protein. Conclusions. The obtained in our study transgenic carrot and celery plants able to express the recombinant thaumatin II gene were characterized by antibacterial activity and increased tolerance to salinity and osmotic stress factors.
Keywords: carrot, celery, thaumatin II, Agrobacterium-mediated transformation, stress tolerance.
Full text: (PDF, in English)

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