Biopolym. Cell. 2015; 31(4):243-248.
http://dx.doi.org/10.7124/bc.0008E7
Reviews
CRISPR/Cas9 technology for targeted genome editing
1, 3Lomov N. A., 1, 3Borunova V. V., 1, 2, 3Rubtsov M. A.
  1. M. V. Lomonosov Moscow State University
    Leninskie Gory, 1/12, Moscow, Russian Federation, 119991
  2. I. M. Sechenov First Moscow State Medical University,
    8, Trubetskaya Str. Moscow, Russian Federation, 119991
  3. LIA 1066 French-Russian Joint Cancer Research Laboratory
    Villejuif, France–Moscow, Russian Federation

Abstract

CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are the segments of prokaryotic DNA containing short repeats in its nucleotide sequence. Today we know that this is a bacterial protection system against viral DNA. The molecular components of CRISPR/Cas9 system have been used for a gene editing in eukaryotes since 2013. But as any other method it also has the limitations and drawbacks. Here we are going to review the history of CRISPR biology and to discuss the possibilities that this new technology provides to researchers as well as the prospects for its use in the medical research and treatment.
Keywords: CRISPR/Cas9, genome targeting, genome editing, personalized therapy, chromosomal translocations, DNA repair
Full text: (PDF, in English)

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