Biopolym. Cell. 2015; 31(3):200-208.
http://dx.doi.org/10.7124/bc.0008E1
Molecular and Cell Biotechnologies
Influence of rat progenitor neurogenic cells supernatant on glioma 101.8 cells in vitro
1Liubich L. D., 1Semenova V. M., 1Stayno L. P.
  1. SI "A. P. Romodanov Institute of Neurosurgery NAMS of Ukraine"
    32, Platona Mayborody Str., Kiev, 04050

Abstract

Aim. To evaluate the influence of the rat progenitor neurogenic cells supernatant (RPNS) on the transplantable rat malignant brain glioma cells (strain 101.8) under conditions of cultivation. Methods. Primary cultures were obtained from glioma 101.8 fragments (n = 12) and intact brain of newborn rats (n = 9). RPNS was received from neurogenic cell suspensions of fetal rat brain on 8–11th (E8-11) and 12–16th (E12-16) days of gestation. Results: RPNS (E8-11) as well as RPNS (E12-16) showed a cytotoxic effect on the glioma 101.8 cells in short-term cultures, the level of which was dose-dependent and intensified with increasing duration of incubation. RPNS (E12-16) had a more pronounced cytotoxic action on the cells of glioma 101.8 compared with RPNS (E8-11). The cytotoxic index (CI) of RPNS (E12-16) on the glioma 101.8 cells was significantly higher than CI determined in cell suspensions of normal rat brain (CI was (91.99 ± 2.37) % and (22.9 ± 4.97) % respectively over 48 h incubation with RPNS). After RPNS (E8-11) influence on the glioma 101.8 primary cultures the signs of dose-dependent cytotoxic effects were observed: the thinning of growth areas, appearance of dystrophic and necrobiotic changes in tumor cells and decreasing of a mitotic index. These features were strengthened under the RPNS (E12-16) influence. Conclusions. Fetal RPNS showed dose-dependent cytotoxic and antiproliferative effects on the cultivated glioma 101.8 cells, which were intensified with the increasing of rat brain gestational age and lengthening of the incubation duration. A prerequisite for such effects is likely the NPC ability to produce the substances with antitumor activity.
Keywords: progenitor neurogenic cells, rat fetal brain, supernatant, glioma 101.8, cytotoxic index, mitotic index
Full text: (PDF, in English)

References

[1] Ahmed AU, Ulasov IV, Mercer RW, Lesniak MS. Maintaining and loading neural stem cells for delivery of oncolytic adenovirus to brain tumors. Methods Mol Biol. 2012;797:97-109.
[2] Bovenberg MS, Degeling MH, Tannous BA. Advances in stem cell therapy against gliomas. Trends Mol Med. 2013;19(5):281-91.
[3] Kim SU. Neural stem cell-based gene therapy for brain tumors. Stem Cell Rev. 2011;7(1):130-40.
[4] Staflin K, Lindvall M, Zuchner T, Lundberg C. Instructive cross-talk between neural progenitor cells and gliomas. J Neurosci Res. 2007;85(10):2147-59.
[5] Klassen HJ, Imfeld KL, Kirov II, Tai L, Gage FH, Young MJ, Berman MA. Expression of cytokines by multipotent neural progenitor cells. Cytokine. 2003;22(3-4):101-6.
[6] Liu J, G?therstr?m C, Forsberg M, Samuelsson EB, Wu J, Calzarossa C, Hovatta O, Sundstr?m E, ?kesson E. Human neural stem/progenitor cells derived from embryonic stem cells and fetal nervous system present differences in immunogenicity and immunomodulatory potentials in vitro. Stem Cell Res. 2013;10(3):325-37.
[7] Chen HC, Ma HI, Sytwu HK, Wang HW, Chen CC, Liu SC, Chen CH, Chen HK, Wang CH. Neural stem cells secrete factors that promote auditory cell proliferation via a leukemia inhibitory factor signaling pathway. J Neurosci Res. 2010;88(15):3308-18.
[8] Lisyanyi NI, Oleynik GM, Markova OV, Semenova VM, Nosov AT The brain cells effect on tumors growth under the kidney capsule in vivo In.: The immune system of the brain Ed. by Lisyanyi N. I. Kyiv, VIPOL. 1999; 116–35.
[9] Grydina NYa, Semyonova VM, Stayno LP, Pogrebnoy PV, Markeeva NV, Shostak EA, Kavsan VM, Rozumenko VD. Transplantation influence of embryonic nervous tissue that contains genetically modified cells on rat glioma 101.8 growth. Transplantologia. 2004; 4:267–9.
[10] Lisyany NI, Lyubych LD, Hohlov AG. Study of progenitor neural cells (NC) antitumor action in experimental rat glioma. Immunol Allergol 2008; 3:61–6.
[11] Osterman LA. Methods for studying proteins and nucleic acids. Moskow: MCNMO, 2002. 248 p.
[12] Guidelines for the cultivation of neural tissue. Methods. Applicances. Problems. Eds. VP Bozhkova, LA Brezhe-stovsky, V Buravlev Moskow: Nauka, 1988. 318 p.
[13] Kaminska B, Kocyk M, Kijewska M. TGF beta signaling and its role in glioma pathogenesis. Adv Exp Med Biol. 2013;986:171-87.
[14] Zhang J, Yang W, Zhao D, Han Y, Liu B, Zhao H, Wang H, Zhang Q, Xu G. Correlation between TSP-1, TGF-? and PPAR-? expression levels and glioma microvascular density. Oncol Lett. 2014;7(1):95-100.
[15] Dubrovska AM, Souchelnytskyi SS. Low-density microarray analysis of TGF?1-dependent cell cycle regulation in human breast adenocarcinoma MCF7 cell line. Biopolym Cell. 2014; 30(2):107–17.
[16] Binder DK, Scharfman HE. Brain-derived neurotrophic factor. Growth Factors. 2004;22(3):123-31.