Biopolym. Cell. 2014; 30(5):394-399.
Molecular and Cell Biotechnologies
Comparative study on influence of fetal bovine serum and serum of adult rat on cultivation of newborn rat neural cells
1, 2Sukach A. N., 1Shevchenko M. V., 2Liashenko T. D.
- Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 - G. S. Skovoroda Kharkiv National Pedagogical University
29, Artema Str., Kharkiv, Ukraine, 61002
Abstract
Aim. To study the influence of fetal bovine serum and serum of adult rats on behavior of newborn rat isolated neural cells during their cultivation in vitro. Methods. The isolation of neural cells from neonatal rat brain. The determination of the dynamics of cellular monolayer formation. Immunocytochemical staining of cells for β-tubulin III, nestin and vimentin. Results. It has been determined that the addition of serum of adult rats to the cultivation medium creates more favorable conditions for survival, attachment and spread of differentiated, and proliferation of the stem/progenitor neural cells of newborn rats during cultivation in vitro compared with the fetal bovine serum. Conclusions. Using the serum of adult rats is preferable for the cultivation of isolated neural cells of newborn rats compared with the fetal bovine serum.
Keywords: newborn rat neural cells, newborn rats, cultivation, fetal bovine serum, adult rats serum
Full text: (PDF, in English)
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