Biopolym. Cell. 2014; 30(1):25-28.
http://dx.doi.org/10.7124/bc.00087C
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Structure and function of oncogene-transfected immortal cells
1Kavsan V. M., 2Kulagova T. A., 3Kuznetsova T. A., 3Semenkova G. N., 1Stepanenko A. A., 4Vassetzky Y. S.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Belarusian State University
    4, Nezavisimosti avenue, Minsk ave., Republic of Belarus, 220030
  3. Pirogov Russian National Research Medical University
    1, Ostrovitianov Str., Moscow, Russian Federation, 117997
  4. CNRS UMR 8126, Universit Paris-Sud 11, Institut Gustave Roussy
    114, rue Edouard Vaillant, Villejuif, France, 94805

Abstract

Previously we have characterized a new oncogene CHI3L1, overexpressed in the glioblastoma and the malignant 293_CHI3L1 cells, stably producing the CHI3L1 angiogenic oncoprotein. The 293_CHI3L1 cells proliferated faster and acquired a higher ability for anchorage-independent growth. Here, we report the atomic force microscopy data and functional characteristics of these cells. The constitutive CHI3L1 expression leads to the increased resistance to the damages by oxidative substances and promotes the chromosome instability in the 293 cells. According to the data of the last clinical investigations, anti-cancer therapy should be targeted not at the individual genes, but at the pathological effects they caused. We propose a complex treatment of gliomas including multi-target inhibitors, which can be delivered to the brain tumor by a specific nanoparticle vector.
Keywords: oncogene CHI3L1, atomic force microscopy, reactive oxygen species (ROS), 293 cells (HEK293), combinatorial chemotherapy, chromosome instability (CIN).
Full text: (PDF, in English)

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