Biopolym. Cell. 1987; 3(6):307-312.
http://dx.doi.org/10.7124/bc.000202
Gene-Engineering Biotechnology
Particularities of cloning of Escherichia coli rpoBC-operon fragments in the pUC plasmids
1Paton E. B., 1Kroupskaya I. V., 1Zhyvoloup A. N.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Recombinant plasmids with inserted BglII E. coli rpoBC-operon fragment, including the whole rplL, rplJ and rpoB-genes and parts of rplA and rpoC-genes are constructed, plasmid pUC19 and cosmid pJC703 being the origin of them. The recombinant plasmid pUCIS with an inserted strong promoter of the rpoBC-operon — PJ as well as recombinant plasmids pUC18 and pUC19 carrying the 2870 bp central fragment of the rpoB-gene are also constructed. Orientations of the cloned fragments are determined for all the recombinant plasmids. It is supposed that strong PJ-promoter including part of the BglII pJC703 fragment determines its orientation when it is inserted into the pUC plasmid.
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