Biopolym. Cell. 2013; 29(6):511-514.
Bioorganic Chemistry
Lepidine Orange derivative as a new dye
for sensitive fluorescent detection of DNA
1Didan Yu. V., 2Kryvorotenko D. V., 2Negrutska V. V., 2Dubey I. Ya.
- Educational and Scientific Center "Institute of Biology",
Taras Shevchenko National University of Kyiv
64/13, Volodymyrska Str., Kyiv, Ukraine, 01601 - Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
Aim. To study new cyanine dye LO-7 as a reagent for dsDNA visualization in electrophoretic gels. Methods. Gel electrophoresis, restriction, fluorescence detection, mobility shift assay. Results. LO-7 binds to DNA to form stable highly fluorescent complexes. As little as 80 pg DNA can be detected in LO-7-stained agarose gel using a laser scanner, and 0.3 ng with UV-transilluminator. This sensitivity is several times higher than can be achieved with ethidium bromide, and close to that of SYBR Green I. Conclusions. LO-7 belongs to the most efficient stains for dsDNA visualization and thus can be used in bioanalytical applications where high sensitivity is required.
Keywords: fluorescent dyes, cyanines, gel electrophoresis, nucleic acids, detection
Full text: (PDF, in English)
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