Biopolym. Cell. 2012; 28(6):441-448.
http://dx.doi.org/10.7124/bc.000134
Molecular and Cell Biotechnologies
Potentiality of application of the conductometric L-arginine biosensors for the real sample analysis
1, 2Saiapina O. Y., 1, 3Dzyadevych S. V., 2Jaffrezic-Renault N.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Laboratory of Analytical Sciences, University Claude Bernard
    Lyon 1, 43, Boulevard du 11 Novembre 1918, Villeurbanne Cedex, France, 69622
  3. Institute of High Technologies,
    Taras Shevchenko National University of Kyiv
    2, korp.5, Pr. Akademika Hlushkova, Kyiv, Ukraine, 03022

Abstract

Aim. To determine an influence of serum components on the L-arginine biosensor sensitivity and to formulate practical recommendations for its reliable analysis. Methods. The L-arginine biosensor comprised arginase and urease co-immobilized by cross-linking. Results. The biosensor specificity was investigated based on a series of representative studies (namely, through urea determination in the serum; inhibitory effect studies of mercury ions; high temperature treatment of sensors; studying the biosensor sensitivity to the serum treated by enzymes, and selectivity studies). It was found that the response of the biosensor to the serum injections was determined by high sensitivity of the L-arginine biosensor toward not only to L-arginine but also toward two other basic amino acids (L-lysine and L-histidine). Conclusions. A detailed procedure of optimization of the conductometric biosensor for L-arginine determination in blood serum has been proposed.
Keywords: L-arginine, conductometric biosensors, serum, optimization procedure
Full text: (PDF, in English)

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