Biopolym. Cell. 2012; 28(4):302-305.
http://dx.doi.org/10.7124/bc.000064
Methods
Optimization of tumor cell culture conditions in soft agar for subsequent immunohistochemical analysis
1Khoruzhenko A. I.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. The aim of this work is to optimize conditions of malignant cells cultivation in soft agar for subsequent immunohistochemical analysis of formed three-dimensional colonies. Methods. Cultivation of breast carcinoma cell line MCF-7 in soft agar, immunohistochemical and im- munofluorescence detection of epithelial antigen and mTOR kinase in cultured cells. Results. We describe a methodical approach to the cultivation of cells in soft agar, which allows to carry out morphological, morphometric and immunochemical analysis of the studied cells. Conclusions. The proposed method provides an additional characteriza tion of cells growing in soft agar, which will be useful in basic research and in evaluation of the effectiveness of anticancer drugs.
Keywords: soft agar, evaluation of colony formation, three-dimensional culture of malignant cells
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