Biopolym. Cell. 2012; 28(3):229-233.
http://dx.doi.org/10.7124/bc.000053
Bioorganic Chemistry
Spectral-luminescent properties of derivatives of squaraine probes at interaction with the isolated rat liver cells
1Tkachova T. N., 1Kavok N. S., 1Borovoy I. A., 1Obukhova O. M., 1Klimov S. A., 1Malyukin Yu. V.
  1. Institute for Scintillation Materials, NAS of Ukraine
    60, Lenin Ave., Kharkiv, Ukraine, 61001

Abstract

Aim. To find out approaches for studying cellular functional state using new fluorescent squaraine probes. Methods. Dependence of fluorescence parameters ofsquaraine and polymethine dyes on the properties of their microenvironment in biomembranes wasstudied by microspectrofluorimetry. The quantitative microfluorimetry of single cells was applied for the evaluation of probes accumulation in cells and for investigation of dynamics of dyes photobleaching. Results. The data obtained evidence that a chromophoric part of the squaraine dyes molecules is mainly localized in a more polar microenvironment in biomembranes, as compared to the cationic polymethine probes. Conclusions. Chromophore of anionic squaraine probe in native membranes is located in the upper membrane layer in the region of polar phospholipids heads, while the cationic probes can penetrate deeper into the membrane. The anionic squaraine probe SqSC4 was determined as the most photostable dye among the investigated probes.
Keywords: emission spectra, squaraine probes, polymethine probes, biomembranes
Full text: (PDF, in Russian)

References

[1] Ioffe V. M., Gorbenko G. P., Tatarets A. L., Patsenker L. D., Terpeching E. A. Examining protein-lipid interactions in model systems with a new squarylium fluorescent dye J. Fluoresc 2006 16, N 4:547–554.
[2] Jisha V. S., Arun K. T., Hariharan M., Ramaiahah D. Site-selective interactions: squaraine dye-serum albumin complexes with enchanced fluorescence and triplet yields J. Phys. Chem. B 2010 114, N 17:5912–5919.
[3] Arun K. T., Ramaiah D. Near-infrared fluorescent probes: synthesis and spectroscopic investigations of a few amphiphilic squaraine dyes J. Phys. Chem. A 2005 109, N 25:5571–5578.
[4] Santos P. F., Reis L. V., Almeida P., Oliveira A. S., Vieira Ferreira L. F. Singlet oxygen generation ability of squarylium cyanine dyes J. Photochem. Photobiol. A: Chemistry 2003 160, N 3 P. 159–161.
[5] Gayathri Devi D., Cibin T. R., Ramaiah D., Abraham A. Bis(3, 5-diiodo-2,4,6-trihydroxyphenyl) squaraine: A novel candidate in photodynamic therapy for skin cancer in vivo J. Photochem. Photobiol. B 2008 92, N 3:153–159.
[6] Oswald B., Patsenker L., Duschl J., Szmacinski H., Wolfbeis O. S., Terpetschnig E. Synthesis, spectral properties, and detection limits of reactive squaraine dyes, a new class of diode laser compatible fluorescent protein labels Bioconjug. Chem 1999 10, N 6:925–931.
[7] Kanaeva I. P., Karjakin A. V., Alenicheva T. V., Burmantova G. A., Alimov G. A., Archakov A. I., Zenkevich G. D. Respiration and oxidative phosphorylation in isolated liver cells Cytology 1975 17, N 5:545–551.
[8] Vigo J., Salmon J. M., Lahmy S., Viallet P. Fluorescent image cytometry: from qualitative to quantitative measurements Anal. Cell. Pathol 1991 3, N 3:145–165.
[9] Dobretsov GE. Fluorescent probes in the study of cell membranes and lipoproteins. Moscow: Nauka, 1989. 277 p.
[10] Malyukin Y. V., Borovoy I. A., Kavok N. S., Gerashchenko A. V., Pogrebnyak N. L., Efimova S. L., Lebedenko A. N. Accumulation of oxacarbocyanine dyes with different alkyl chain length in bone marrow cells and hepatocytes Biophysics 2007 52, N 4:667–677.