Biopolym. Cell. 2011; 27(5):343-346.
http://dx.doi.org/10.7124/bc.00011F
Stimulation of transient versus sustained ERK1/2 phosphorylation by relative chitinase-like proteins CHI3L1 and CHI3L2 correlates with different kinase localization and biological outcome
1Areshkov P. O., 1Avdieiev S. S., 1Iershov A. V., 1Kavsan V. M.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. To determine 293 and U373 cell response and ERK1/2 activation profile after CHI3L1 or CHI3L2 treatment. Methods. Specific activation and localization of ERK1/2 kinases after CHI3L1 or CHI3L2 addition to unsupplemented cell medium were evaluated by Western blots, immunofluorescence and confocal microscopy. To determine whether CHI3L1 or CHI3L2 can enhance mitogenesis, [3H]thymidine incorporation in cellular DNA was measured. Results. The obtained results show that ERK1/2 phosphorylation was stimulated in both cell types (293 and U373 cells) following addition of CHI3L2 or CHI3L1 in dose- and time-dependent manner. Unexpectedly, in opposite to CHI3L1, the dose-dependent decreasing of measured mitogenesis parameters was observed in 293 and U373 cells. In both cell types the treatment with CHI3L2 gave more sustained than CHI3L1 MAPK-pathway activation with prolonged phospho-ERK1/2 nuclear accumulation in 293 cells. Conclusions. In contrast to the activation of ERK1/2 phosphorylation by CHI3L1 cell treatment, that leads to a proliferative signal, the activation of these kinases by CHI3L2 addition inhibits cell mitogenesis and proliferation in serum starved 293 and U373 cells.
Keywords: chitinase 3-like 1 protein (CHI3L1), chitinase 3-like 2 protein (CHI3L2), MAP kinase, mitogenesis
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