Biopolym. Cell. 2011; 27(5):339-342.
http://dx.doi.org/10.7124/bc.00011E
Brain plasticity of rats exposed to prenatal immobilization stress
1Abrahamyan S. S., 2Meliksetyan I. B., 1Sahakyan I. K., 1Tumasyan N. V., 3Badalyan B. Yu., 1Galoyan A. A.
  1. H. Buniatian Institute of Biochemistry, NAS of Republic of Armenia
    5/1, P. Sevak Str., Yerevan, Republic of Armenia, 0014
  2. L. A. Orbeli Institute of Physiology, NAS of Republic of Armenia
    22, Orbeli Str., Yerevan, Republic of Armenia, 0028
  3. Yerevan State Medical University named after Mkhitar Heratsi, Ministry of Education and Science of Republic of Armenia
    2, Koryun Str., Yerevan, Republic of Armenia, 0025

Abstract

Aim. This histochemical and immunohistochemical study was aimed at examining the brain cellular structures of newborn rats exposed to prenatal immobilization (IMO) stress. Methods. Histochemical method on detection of Ca2+-dependent acid phosphatase activity and ABC immunohistochemical technique. Results. Cell structures with radial astrocytes marker GFAP, neuroepithelial stem cell marker gene nestin, stem-cells marker and the hypothalamic neuroprotective proline-rich polypeptide PRP-1 (aka Galarmin, a natural cytokine of a common precursor to neurophysin vasopressin associated glycoprotein) have been revealed in several brain regions. Conclusions. Our findings indicate the process of generation of new neurons in response to IMO and PRP-1 involvement in this recovery mechanism, as PRP-1-Ir was detected in the above mentioned cell structures, as well as in the neurons and nerve fibers.
Keywords: rat brain plasticity, prenatal immobilization stress, GFAP-, nestin-, stem cells-, PRP-1-immuno-reactive structures
Full text: (PDF, in English)

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