Biopolym. Cell. 2011; 27(3):206-213.
Molecular Biomedicine
Study on association of the polymorphic variants of ACE (I/D), AT2R1 (A1166C), TNF-α (G308A), MTHFR (C677T) genes and their combinations with the risk of development of perinatal pathology and gestation reduction
1, 3Gorovenko N. G., 1, 2Kyryachenko S. P., 2, 3Rossokha Z. I.
- P. L. Shupik National medical academy of post-graduate education
9, Dorohozhytska Str., Kyiv, Ukraine, 04112 - Reference-center of molecular diagnostic MH of Ukraine
9, Dorohozhytska Str., Kyiv, Ukraine, 04112 - State Organization of Genetic and Regenerative Medicine NAMS Ukraine
67, Vyshhorodska Str., Kyiv, Ukraine, 04114
Abstract
Aim. To study the association of the polymorphic variants of ACE (I/D), AT2R1 (A1166C), TNF-α (G308A), MTHFR (C677T) genes and their combinations with the risk of perinatal pathology and gestation reduction. Methods. The polymorphic variants of genes were analyzed by PCR and RFLP in 235 newborns with severe perinatal pathology and 110 clinically healthy term newborns. Results. An increased risk of severe perinatal pathology was associated with such genotypes: DD and ID (ACE), 1166AC, 1166CC (AT2R1), 677CT (MTHFR), 308AA and 308AG (TNF-α), this risk for homozygotes is almost 2-fold higher than for heterozygotes. Reduction of terms of gestation is associated with the genotype 677TT (MTHFR), and resistance to diseases in the perinatal period – with the genotype II (ACE) and 1166AA (AT2R1), 677CC (MTHFR) and the 308GG (TNF-α), particularly when combined. Conclusions. The identified associations evidence the role of polymorphic variants of ACE, AT2R1, TNF-α, MTHFR genes in the development of severe perinatal pathology and can be used for its early prediction with subsequent correction of treatment.
Keywords: perinatal pathology, polymorphism, ACE, AT2R1, TNF-α, MTHFR genes
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