Biopolym. Cell. 2011; 27(1):66-73.
http://dx.doi.org/10.7124/bc.000084
Molecular Biomedicine
Study of possible role of CYP1A1, GSTT1, GSTM1, GSTP1, NAT2 and ADRB2 genes polymorphisms in bronchial asthma development in children
1Tatarskyy P. F., 4Chumachenko N. G., 1, 2Kucherenko A. M., 1, 2Gulkovskyi R. V., 3Arabskaya L. P., 3Smirnova O. A., 3Tolkach S. I., 3Antipkin Yu. G., 1Livshits L. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Taras Shevchenko University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  3. State Enterprise "Institute of Pediatrics, Obstetrics and Gynecology" NAMS of Ukraine
    8, Manuil'skoho St., Kyiv, Ukraine, 04050
  4. Town hospital N 9
    72, Anoshkina St., Dniprodzerzhyns'k, Ukraine, 51934

Abstract

Aim. To study the association of polymorphisms of the enzymes genes CYP1A1 (T6235C), first phase, and NAT2 (C481T, G590A, G857A), GSTM1 («0»), GSTT1 («0») and GSTP1 (A313G), second phase of the detoxication system, as well as the ADRB2 (C79G) gene variants with the development of bronchial asthma in children. Methods. Polymorphic variants were analyzed using PCR followed by RFLP analysis in 86 healthy individuals and in 114 patients with clinical diagnosis of bronchial asthma. Results. The frequency of gene polymorphic variants of the enzymes of first and second phases of detoxification system as well as the ADRB2 gene was established in the children with bronchial asthma and healthy individuals. Conclusions. ?olymorphic variants of the genes NAT2 (481T), GSTP1 (313G) and ADRB2 (79G) and their combinations in genotype were observed more frequently in the patients with bronchial asthma comparing to the control group, which indicates their involvement in the pathogenesis of asthma in children.
Keywords: bronchial asthma, detoxication system, 2-adrenoreceptor gene, polymorphism, combined genotype
Full text: (PDF, in English) (PDF, in Ukrainian)

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