Biopolym. Cell. 2009; 25(6):445-450.
http://dx.doi.org/10.7124/bc.0007F6
Structure and Function of Biopolymers
Cloning, expression and purification of tRNAPro from bacteria Enterococcus faecalis
1Boyarshin K. S., 1Kriklivyi I. A., 1Yaremchuk A. D., 1Tukalo M. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. To elaborate the method of expression and purification of bacteria Enterococcus faecalis tRNAPro transcript. Methods. tRNA, co-expressed in vitro with cis-hydrolytical ribozyme, was purified by high performance liquid chromatography using anion-exchange chromatographic column. Results. A satisfactory yield of high purity preparation was obtained. A transcript of tRNAPro exhibits acceptor activity in aminoacylation reaction. Conclusions. The method developed may be introduced in laboratory practice including the obtaining of other tRNAs.
Keywords: RNA, transcript, gene expression in vitro, high performance liquid chromatography
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