Biopolym. Cell. 2009; 25(4):272-278.
http://dx.doi.org/10.7124/bc.0007E6
Molecular and Cell Biotechnologies
Enzyme conductometric biosensor for maltose determination
1, 2Pyeshkova V. M., 1Saiapina O. Y., 1Soldatkin O. O., 1Dzyadevych S. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01601

Abstract

Aim. To develop enzyme conductometric biosensor for maltose determination. Methods. A conductometric transducer consisting of two gold pairs of electrodes was applied. Three-enzyme membrane (glucose oxidase, mutarotase, α-glucosidase) immobilized on the surface of the conductometric transducer was used as a bioselective element. Results. A linear range of maltose conductometric biosensor was from 0,002 mM to 1 mM for glucose and maltose detection. The time of maltose analysis in solution was 1–2 minutes. The dependence of biosensor responses to substrate on pH, ionic strength, and buffer capacity of work solution was studied. The data of biosensor selectivity are presented. The developed conductometric biosensor is characterized by high operational stability and signal reproducibility. Conclusion. The enzyme conductometric biosensor for maltose determination has been developed. The analytical characteristics of the maltose biosensor were investigated. The proposed method could be used in food industry to control and optimize production.
Keywords: conductometric biosensor, maltose, mutarotase, glucose oxidase, α-glucosidase
Full text: (PDF, in English) (PDF, in Ukrainian)

References

[1] Magomedov G. O. Technology of caramel Kiev, 2008 209 p.
[2] David A. B. Dictionary of food and nutrition Oxford: Univ. press, 2005 608 p.
[3] Filipiak M., Fludra K., Gosciminska E. Enzymatic membranes for determination of some disacchrides by means of oxygen electrode Biosensors and Bioelectronics 1996 11, N 4 :355–364.
[4] Gondo S., Kim C., Hirata S., Morishita M. Studies on dynamic behavior of the biosensor based on immobilized glucoamylase-glucose oxidase membrane Biosensors and Bioelectronics 1997 12, N 5:395–401.
[5] Ge F., Zhang X. E., Zhang P., Zhang X. M. Simultaneous determination of maltose and glucose using a screen-printed electrode system Antonie Van Leeuwenhoek 1997 71, N 4:345–351.
[6] Menzel C., Lerch T., Scheper T., Schugerl K. Development of biosensors based on an electrolyte isolator semiconductor (EIS)-capacitor structure and their application for process monitoring. Part I. Development of the biosensors and their characterization Anal. Chim. Acta 1995 317, N 1–3:259–264.
[7] D. Pfeiffer, F. Scheller, M. Janchen, K. Bertermann. Glucose oxidase bienzyme electrodes for ATP, NAD+, starch and disaccharides. Biochimie. 1980;62(8-9):587-93.
[8] Mori T., Motonaga T., Okahata Y. Cast films of lipid-coated enzymes as selective sensors for disaccharides Colloids and Surfaces A: Physicochemical and Engineering Aspects 1999 146:387–395.
[9] Kullick T., Beyer M., Henning J., Lerch T., Quack R., Zeitz A., Hitzmann B., Scheper T., Schugerl K. Application of enzymefield effect transistor sensor arrays as detectors in a flowinjection analysis system for simultaneous monitoring of medium components. Part I. Preparation and calibration Anal. Chim. Acta 1994 296:263–269.
[10] Zajoncova L., Jilek M., Beranova V., Pec P. A biosensor for the determination of amylase activity. Biosens Bioelectron. 2004;20(2):240-5.
[11] Kullick T., Bock U., Schubert J., Scheper T., Schugerl K. Application of enzyme-field effect transistor sensor arrays as detectors in a flow-injection analysis system for simultaneous monitoring of medium components. Part II. Monitoring of cultivation processes Anal. Chim. Acta 1995 300:25–31.
[12] Aoki K., Uchida H., Katsube T., Ishimaru Y., Iida T. Integration of bienzymatic disaccharide sensors for simultaneous determination of disaccharides by means of light addressable potentiometric sensor Anal. Chim. Acta 2002 471:3– 12.
[13] Tessema M., Ruzgas T., Gorton L., Ikeda T. Flow injection amperometric determination of glucose and some other low molecular weight saccharides based on oligosaccharide dehydrogenase mediated by benzoquinone systems Anal. Chim. Acta 1995 310:161–171.
[14] Sun C., Zhang X., Jiang D., Gao Q., Xu H., Sun Y., Zhang X., Shen J. Electrocatalytic oxidation of carbohydrates at a molecular deposition film electrode based on water-soluble cobalt phthalocyanine and its application to flow-through detection J. Electroanal. Chem 1996 411, N 1–2:73– 78.
[15] Dzyadevych S. V., Soldatkin O. P. Conductometric method of measurements in enzyme analysis. Ukr Biokhim Zh. 1994; 66(4):30–42.
[16] Dzyadevych S. V. Conductometric enzyme biosensors theory, technology and application Biopolym. Cell 2005 21, N 2:91–106.
[17] Dzyadevych S. V., Shulga A. A., Patskovsky S. V., Arkhipova V. N., Soldatkin A. P., Strikha V. I. Thin-films conductometric transducer for enzyme biosensors. Rus. J. Electrochem. 1994; 30(8):887–891.
[18] Pyeshkova V. M., Saiapina O. Y., Soldatkin O. O., Kukla O. L., Dzyadevych S. V. Enzyme conductometric biosensor for determination of lactose. Biotechnology. 2008; N 4:76–84.