Biopolym. Cell. 2008; 24(3):238-245.
Biomedicine
Changes in the content of molecular chaperone Hsp60 in heart tissue at dilated cardiomyopathy
1Kapustian L. M., 1Rozhko O. T., 1Bobyk V. I., 1Kroupska I. V., 2Riabenko D. V., 3Khozhaenko Yu. S., 3Gurtovyy V. A., 3Usenko V. S., 1Sidorik L. L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Scientific Center "M. D. Strazhesko Institute of Cardiology, MAS of Ukraine"
    5, Narodnogo Opolchennya Str., Kyiv, Ukraine, 03151
  3. Pathomorphological Laboratory "BIONTEK"
    52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000

Abstract

The changes of Hsp60 content in heart tissue at dilated cardiomyopathy (DCM) were investigated. An increase in Hsp60 level was observed in human hearts affected by DCM as well as in the hearts of mice with disease model similar to human DCM. We observed the increase in Hsp60 level in mitochondria and decrease in cytoplasmic fraction, which could be one of the reasons for apoptotic responce in cardiomyocytes at heart failure associated with chronic stress. The results of immunohystochemical analysis of mice hearts with the model human-like pathology demonstrate heterogeneity in chaperone levels. A considerable increase in Hsp60 staining was observed only in some cardiomyocytes, possibly in those with activated antistress mechanisms of cell defense against chronic stress.
Keywords: molecular chaperone, Hsp60, dilated cardiomyopathy, cardiomyocytes, apoptosis

References

[1] Reeve JL, Duffy AM, O'Brien T, Samali A. Don't lose heart--therapeutic value of apoptosis prevention in the treatment of cardiovascular disease. J Cell Mol Med. 2005;9(3):609-22.
[2] Gupta S, Knowlton AA. HSP60, Bax, apoptosis and the heart. J Cell Mol Med. 2005;9(1):51-8.
[3] Lin KM, Lin B, Lian IY, Mestril R, Scheffler IE, Dillmann WH. Combined and individual mitochondrial HSP60 and HSP10 expression in cardiac myocytes protects mitochondrial function and prevents apoptotic cell deaths induced by simulated ischemia-reoxygenation. Circulation. 2001;103(13):1787-92.
[4] Gupta S, Knowlton AA. Cytosolic heat shock protein 60, hypoxia, and apoptosis. Circulation. 2002;106(21):2727-33.
[5] Kirchhoff SR, Gupta S, Knowlton AA. Cytosolic heat shock protein 60, apoptosis, and myocardial injury. Circulation. 2002;105(24):2899-904.
[6] Shan YX, Liu TJ, Su HF, Samsamshariat A, Mestril R, Wang PH. Hsp10 and Hsp60 modulate Bcl-2 family and mitochondria apoptosis signaling induced by doxorubicin in cardiac muscle cells. J Mol Cell Cardiol. 2003;35(9):1135-43.
[7] Bobyk V. I., Ryabenko D. V., Sergienko O. V., Trunina I. V., Fedorkova O. M., Morozova L. M., Sidorik L. L. Experimental model of autoimmune myosin-induced myocardium injury. Biopolym. Cell. 2007; 23(2):115-121
[8] Ryabenko D. V., Sidorik L. L., Bobyk V. I., Sergiyenko O. V., Fedorkova O. M., Trunina I. V., Matsuka G. KH. Morphological features of autoimmune myocardial damage due to various myocardial human antigens: a comparative experimental study. Ukrainian journal of rheumatology. 2000; 2:55–60.
[9] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[10] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[11] Kapustian L. N., Kyyamova R. G., Gryshkova V. S., Terentiev A. G., Filonenko V. V., Sidorik L. L. Obtaining recombinant chaperon CroEL and its immunological cross-reactivity with Hsp60. Biopolym. Cell. 2006; 22(2):117-120
[12] Immunocytochemical methods and protocols. Ed. C. L. Javois New York: HumanapPress, 1999 233 p.
[13] Mize RR. Quantitative image analysis for immunocytochemistry and in situ hybridization. J Neurosci Methods. 1994;54(2):219-37.
[14] Maron BJ, Towbin JA, Thiene G, Antzelevitch C, Corrado D, Arnett D, Moss AJ, Seidman CE, Young JB; American Heart Association; Council on Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomics and Translational Biology Interdisciplinary Working Groups; Council on Epidemiology and Prevention. Contemporary definitions and classification of the cardiomyopathies: an American Heart Association Scientific Statement from the Council on Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomics and Translational Biology Interdisciplinary Working Groups; and Council on Epidemiology and Prevention. Circulation. 2006;113(14):1807-16.
[15] Knowlton AA, Kapadia S, Torre-Amione G, Durand JB, Bies R, Young J, Mann DL. Differential expression of heat shock proteins in normal and failing human hearts. J Mol Cell Cardiol. 1998;30(4):811-8.
[16] Latif N, Taylor PM, Khan MA, Yacoub MH, Dunn MJ. The expression of heat shock protein 60 in patients with dilated cardiomyopathy. Basic Res Cardiol. 1999;94(2):112-9.
[17] Sabbah HN, Sharov V, Riddle JM, Kono T, Lesch M, Goldstein S. Mitochondrial abnormalities in myocardium of dogs with chronic heart failure. J Mol Cell Cardiol. 1992;24(11):1333-47.
[18] Shan YX, Yang TL, Mestril R, Wang PH. Hsp10 and Hsp60 suppress ubiquitination of insulin-like growth factor-1 receptor and augment insulin-like growth factor-1 receptor signaling in cardiac muscle: implications on decreased myocardial protection in diabetic cardiomyopathy. J Biol Chem. 2003;278(46):45492-8.
[19] Lai HC, Liu TJ, Ting CT, Yang JY, Huang L, Wallace D, Kaiser P, Wang PH. Regulation of IGF-I receptor signaling in diabetic cardiac muscle: dysregulation of cytosolic and mitochondria HSP60. Am J Physiol Endocrinol Metab. 2007;292(1):E292-7.
[20] Gupta S, Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007;292(6):H3052-6.