Biopolym. Cell. 2008; 24(1):28-34.
http://dx.doi.org/10.7124/bc.00078D
Cell Biology
Induction of apoptosis and necrosis in leukemic cells by purified IgG of blood serum of mice which were fed with cattle brain for a long time
1Kozak M. R., 1Vlizlo V. V., 2Kit Y. Y., 2Stoika R. S.
  1. Institute of Animal Biology, NAAS of Ukraine
    38, Stusa Str., Lviv, Ukraine, 79034
  2. Institute of Cell Biology, NAS of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005

Abstract

IgG preparations of mice under study were tested for their ability to induce death of cells of L1210 line of murine leukemia. The highest level of cytotoxic activity was found at affecting cells with IgG of mice that were additionally fed with cattle brain. It was shown that the test cells were dying by both apoptosis (typical signs are destruction of DNA, revealed by the method of DNA-comets, condensation and fragmentation of chromatin, discovered by fluorescent microscopy of cells, stained with Hoechst 33342) and necrosis. The latter was estimated taking into account the level of lactate dehydrogenase activity, which appeared in cultural medium of L1210 cells, and by light microscopy after cell staining according to Romanovsky-Gimza.
Keywords: ration, immunoglobulins, cytotoxic activity, apoptosis, necrosis
Full text: (PDF, in English) (PDF, in Ukrainian)

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