Biopolym. Cell. 2007; 23(5):386-390.
http://dx.doi.org/10.7124/bc.000777
Structure and Function of Biopolymers
Characterization of physical properties of two isoforms of translation elongation factor 1A
1Novosylna A. V., 2Timchenko A. A., 2Tiktopulo E. I., 2Serdyuk I. N., 1Negrutskii B. S., 1El'skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Protein Research, Russian Academy of Sciences
    Pushchino, Moscow Region, Russian Federation, 142290

Abstract

Two tissue-specific isoforms of mammalian translation elongation factor 1A, eEF1A1 and eEF1A2, are 98 % similar. However, some of their functions in the organism are different which in some cases could lead to the induction of carcinogenesis. We supposed that slight difference of primary sequences may cause significant differences of spatial structures of eEF1A isoforms affecting, in its turn, the ability of one or another isoform to interact with protein partners. The differential scanning microcalorimetry and circular dichroism in «near» and «far» UV regions were used to determine that potentially oncogenic eEF1A2 isoform possesses a more compact spatial organization than eEF1A1, and the differences are revealed at the levels of both secondary and tertiary structure of proteins.
Keywords: translation elongation factor eEF1A1, protein biosynthesis, differential scanning microcalorimetry, circular dichroism
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