Biopolym. Cell. 2007; 23(4):324-331.
Cell Biology
Genetic polymorphism of the maize somaclonal lines derived from P346 line
1Maidanyuk D. M., 1Andreev I. O., 1Spiridonova K. V., 1Kunakh V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Some distinctions between the inbred P346 line and five somaclonal Zea mays lines (UKCh-5, UKCh-6, UKCh-7, UKCh-8, and UKCh-9) obtained from it via tissue culture in vitro have been found through RAPD analysis. The somaclonal lines were shown to differ from each other by the magnitude of genetic distances from the original P346 line as well as by the level of intraline heterogeneity. Positive interrelationship was revealed between these indices. The subset of polymorphic amplicons which distinguish the somaclonal lines from the original one proved to be common for some of them. The results obtained along with the earlier studies on the individual phenotypic selection and genetic traits demonstrated that the employment of the tissue culture in vitro together with the appropriate selection methods allow attaining desirable genetic changes.
Keywords: maize, tissue culture in vitro, somaclonal lines, RAPD analysis, genome rearrangements


[1] Dowswell CR. Maize in the world economy. New York: Westview press Inc., 1996. 288 p.
[2] Kozubenko LV, Gur'eva IA. Breeding maize for earliness. Kharkov: Urozhay, 2000; 240 p.
[3] Jain SM. Tissue culture-derived variation in crop improvement. Euphytica. 2004; 118(2): 153-66.
[4] Kunakh VA. Biotechnology of medical plants. Genetic, physiological and biochemical basis Kyiv: Logos, 2005 730 p.
[5] Checheneva TN, Gur'eva IA. Comparative study somaclonal inbred maize lines for quantitative traits. Genetics in Ukraine at the turn of the millennium. K.: Logos, 2001. Vol 1:586-9.
[6] Checheneva TN. Spontaneous and induced variability of maize in vitro: Thesis.... Dr biol nauk: 03.00.15. Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine. K., 2003. 302 p.
[7] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[8] Rogers SO, Bendich AJ. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol. 1985;5(2):69-76.
[9] Nei M. Genetic distance between populations. Am Nat. 1972. 106(949):283-292.
[10] Yeh FC, Rongcai Y, Boyle T. 1999. POPGENE. Version 1.31. University of Alberta, Edmonton, Canada.
[11] Osipova ES, Koveza OV, Troitskiĭ AV, Dolgikh IuI, Shamina ZB, Gostimskiĭ SA. [Analysis of specific RAPD- and ISSR-fragments in somaclonal maize (Zea mays L.) and development of SCAR markers based on them]. Genetika. 2003;39(12):1664-72.
[12] Maidanyuk DN, Andreev IO, Spiridonova KV, Checheneva TN, Kunakh VA. Genome variaability of maize line Black Mexican Sweet Corn C456 in tissue culture in vitro: RAPD-amalysis results. Visn Ukr Tov Genet Sel. 2006. 4(1): 58-67.
[13] Linacero R, Freitas Alves E, Vbzquez AM. Hot spots of DNA instability revealed through the study of somaclonal variation in rye. TAG Theore Appl Genet. 2000;100(3-4):506–11.
[14] Bogani P, Simoni A, Lio' P, Scialpi A, Buiatti M. Genome flux in tomato cell clones cultured in vitro in different physiological equilibria. II. A RAPD analysis of variability. Genome. 1996;39(5):846-53.
[15] Guo WL, Gong L, Ding ZF, Li YD, Li FX, Zhao SP, Liu B. Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. f., as revealed by ISSR and RAPD markers. Plant Cell Rep. 2006;25(9):896-906.
[16] Kozyrenko MM, Artyukova EV, Lauve LS, Zhuravlev IuN, Reunova GD. The Genetic variability of Panax ginseng callus lines. Biotekhnologiia. 2001;(1):19-26.
[17] Martins M, Sarmento D, Oliveira MM. Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers. Plant Cell Rep. 2004;23(7):492-6.
[18] Lattoo SK, Bamotra S, Sapru Dhar R, Khan S, Dhar AK. Rapid plant regeneration and analysis of genetic fidelity of in vitro derived plants of Chlorophytum arundinaceum Baker--an endangered medicinal herb. Plant Cell Rep. 2006;25(6):499-506.