Biopolym. Cell. 2007; 23(2):93-99.
Molecular and Cell Biotechnologies
The development of DNA-vaccine against classical swine fever
1Pokholenko I. A., 1Ruban T. A., 1Sukhorada O. M., 2Deriabin O. M., 1Tytok T. G., 1Kordium V. A.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 - Institute of Veterinary Medicine, NAAS of Ukraine
30, Donetska Str., Kyiv, Ukraine, 03151
Abstract
The development of a DNA-vaccine against classical swine fever
(CSF) is a perspective direction, because it gives an opportunity to
develop a marker vaccine due to use of a part of protective antigen
molecule, and to induce effectively both cellular and humoral
immune response. In this study a recombinant plasmid, containing
the fragment of E2 gene of CFS virus (CSFV) in eukaryotic
expression vector, has been developed. It has been demonstrated
that the fragment of E2 protein of CSFV is expressed in CHO-Kl
cells from the developed recombinant plasmid pTR-BKneo , and we
suggest that the protein possesses the post-translational modifications. The data obtained are in favor of the created model
DNA-vaccine able to induce humoral immune response to fragment
of E2 protein of CSFV.
Keywords: CSFV, DNA-vaccine, E2 glycoprotein of CSFV, immunization, humoral immune response
Full text: (PDF, in English) (PDF, in Ukrainian)
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