Biopolym. Cell. 2006; 22(3):210-216.
http://dx.doi.org/10.7124/bc.000732
Genome and Its Regulation
Study of transient expression of human apoA-1 gene under control of various promoters in CHO-K1 cells
1Gilchuk Y. M., 1Sukhorada O. M., 1Ruban T. A., 1Toporova O. K., 1Kordyum V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The recombinant plasmids containing the genomic human apoA-1 gene under the transcriptional control of hybrid promoters, namely, the cytomegalovirus immediate early enhancer/chicken β-actin promoter and first intron (CAG promoter) and cytomegalovirus immediate early enhancer/promoter with intron A (hCMVintronA promoter) have been constructed. The transient expression of human apoA-1 gene has been studied in CHO-K1 cells using designed and pTRapo vectors. The expression of the transgene for transfected with pTRapo and pTRapochCMVintronA CHO-K1 cells have been shown.
Keywords: transient expression, apolipoprotein A-1, CHO-K1
Full text: (PDF, in English) (PDF, in Ukrainian)

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