Biopolym. Cell. 2004; 20(3):233-238.
http://dx.doi.org/10.7124/bc.0006AA
Viruses and Cell
Purification of intracellular recombinant human prolactin synthesized in insect cells
1Strokovskaya L. I., 1Meleshko R. A., 1Kikhno I. M., 1Solomko A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Human prolactin (PRL) was expressed in the insect cell culture infected with Autographa californica (Ac) nuclear polyhedrosis virus (NPV) which contained prolactin cDNA under the control of polyhedrin gene promoter. Prolactin was expressed as 6 x His-tagged protein and was purified using Co2+-affinity chromatography. The yield of purified protein reached 5 to 50 mg/l depending on the type of insect cells and circumstances of their cultivation. The recombinant prolactin obtained was biologically active.
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