Biopolym. Cell. 1986; 2(6):311-316.
Structure and Function of Biopolymers
The all-machine synthesis of oligodeoxyribonucleotides by phosphotriester approach using automatic synthesizer «Victory-4M»
1Gryaznov S. M., 2Potapov V. K., 2Gorn V. V., 2Zarytova V. F., 2Sredin Yu. G., 3Potemkin G. A., 1Shabarova Z. A.
  1. M. V. Lomonosov State University
    Moscow, USSR
  2. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  3. Special Design Technological Office of the Special Electronics and Analytical Instrument Engineering, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR


The working regime of new model of automatic synthesizer of eligodeoxyribonucleotides «Victory-4M» applicable for two schemes of phosphotriester synthesis of oligonucleotides in 3'-5' direction has been developed, the nucleotide components being either dissolved in the solvents or immobilized on polymer supports. Both styrene-teflon copolymer and silochrome C-80 were used as polymer supports. The comparative analysis of the results of allmachine synthesis of the series of oligodeoxyribonucleotides has shown, that inspite of the differences in the structure and chemical nature of supports and nucleotide components, the average yield was about 75–80 % per step. These results demonstrated the efficiency of synthesizer and applicability of the both synthetic approaches for automatized synthesis of oligonucleotides.


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