Biopolym. Cell. 1986; 2(2):88-92.
Natural mRNA translation. I. General kinetic analysis of the process of one mRNA species translation
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Steady-state kinetics of natural mRNA translation is theoretically analyzed. Equations are obtained for describing dependence of the total rate of cistron translation on the specific rates of each codon reading. The limited capacity of mRNA for ribosomes is shown to limit the increase of the total translation rate. Vmax depends on the sequence of mRNA codons and specific rates of codon reading. For the polyribosomal system, whose total translation rate is close to Vmax, the dependence of the total rate on specific rates of codon reading may be significantly stronger than that for the monoribosomal system. Thus, polysomal organization of the translation apparatus is suggested to be of importance for providing high susceptibility of the translation system to the regulatory factors.
 Spirin AS, Gavrilova LP. Ribosome. Moscow, Nauka, 1971; 254 p.
 Lengyel P. The process of translation: a bird's-eye view. Ribosomes. New York: Cold Spring Harbor Lab., 1974:13-52.
 Shapvil' F, Enni AL. Protein biosynthesis. Moscow, Mir, 1977; 315 p.
 Ames B., Hartman P. The histidine operon. Cold Spring Harb Symp Quant Biol.. 1963. 28:349-56.
 Matsuka GKh, El'skaya AV, Kovalenko MI, Kornelyuk AI. Transfer ribonucleic acid. Kiev, Naukova dumka, 1976; 219 p.
 Osterman LA. Regulatory function of transfer RNA in protein biosynthesis. Usp Sovrem Biol. 1977;83(1):3-22.
 Volkenstein M. V., Goldstein B. N. A new method for solving the problems of the stationary kinetics of enzymological reactions. Biochim biophys acta. 1966; 115(2):471-477.
 Takanami M., Zubay G. An estimate of the size of the ribosomal site for messenger RNA binding. Proc Natl Acad Sci U S A. 1964;51:834-9.
 Castles JJ, Singer MF. Degradation of polyuridylic acid by ribonuclease II: protection by ribosomes. J Mol Biol. 1969;40(1):1-17.
 Steitz JA, Bryan RA. Two ribosome binding sites from the gene 0-3 messenger RNA of bacteriophages T7. J Mol Biol. 1977;114(4):527-43.