Biopolym. Cell. 2003; 19(4):355-361.
http://dx.doi.org/10.7124/bc.000667
Structure and Function of Biopolymers
The α2-macroglobulin–trypsin complex as a sensitive element of a potentiometric biosensor for mediated quantitative determination of proteinases
1Beloivan O. A., 1Soldatkin A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A possibility of application of the α2-macroglobulin (α2M)–trypsin complex as a sensitive element of a potentiometric biosensor for mediated Quantitative determination of proteinases through their esterase activity at the hydrolysis of Na-benzoyl-L-arginin-ethyl ester (BAEE) has been studied. The α2M–trypsin complex immobilization at pH-FET surface in saturated glutaraldehyde (GA) vapours has been optimized. The selective membrane characteristics have been studied. The optimal conditions of measuring BAEE hydrolysis by the biosensor obtained have been determined. A possibility to detect 0.1–1.0 mM BAEE and trypsin activity within the range of 0.1–30 ill ml has been shown.'A conclusion has been made that the membrane developed is promising as a sensitive element of the potentiometric biosensors.
Full text: (PDF, in Russian)

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