Biopolym. Cell. 2003; 19(2):151-156.
Structure and Function of Biopolymers
Participation of nitrogen bases in the tertiary folding of tRNALeu from Thermus thermophilus
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Reactivity of bases in tRNALeu from T. thermophilus has been studied by chemical modification methods. Obtained results suggest that molecules of tRNALeu have a canonical L-form in solution. Triple interactions (8–14)-21, (13–22)-9 and (15–48)-20a arc observed in the core. The latter two interactions represent the characteristic feature of the class II tRNA tertiary structures. Different reactivity of bases 15 and 21 in tRNALeu and tRNASer from T. thermophilus indicates differences in the core tertiary structure of these tRNAs.
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