Biopolym. Cell. 2002; 18(4):334-339.
http://dx.doi.org/10.7124/bc.000615
Molecular and Cell Biotechnologies
The influence of organic carbon sources on the production of a recombinant protein in Escherichia coli cells
1Slavchenko I. Yu.
  1. PSRS "Biotechnolog"
    150, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

The influence of different carbon sources in growth media on the production of a recombinant protein (IFN) in E. coli cells was investigated. It was ascertained that upon the cultivation of the IFN producer E. coli SG30 (pIF-16) at 37 В°C the carbon sources content in the media may cause either increase or decrease in the yield of target product. The level of recombinant protein production increases when glycerol, rhamnose, fructose, sorbitol are added to the cultivation media, while glucose, maltose, mannitol, xylose decreases it. The negative influence of these carbon sources was shown to be temperature-dependent and followed by strong reduction in pH of medium. Possible mechanisms of influence of carbon sources in the media on the production of a recombinant protein in E. call cells are discussed.
Full text: (PDF, in Russian)

References

[1] Schemidt RA, Qu J, Williams JR, Brusilow WS. Effects of carbon source on expression of F0 genes and on the stoichiometry of the c subunit in the F1F0 ATPase of Escherichia coli. J Bacteriol. 1998;180(12):3205-8.
[2] Park SJ, Gunsalus RP. Oxygen, iron, carbon, and superoxide control of the fumarase fumA and fumC genes of Escherichia coli: role of the arcA, fnr, and soxR gene products. J Bacteriol. 1995;177(21):6255-62.
[3] Park SJ, Cotter PA, Gunsalus RP. Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability. J Bacteriol. 1995;177(22):6652-6.
[4] Nancib N, Branlant C, Boudrant J. Metabolic roles of peptone and yeast extract for the culture of a recombinant strain of Escherichia coli. J Ind Microbiol. 1991;8(3):165-9.
[5] Chagneau C, Heyde M, Alonso S, Portalier R, Laloi P. External-pH-dependent expression of the maltose regulon and ompF gene in Escherichia coli is affected by the level of glycerol kinase, encoded by glpK. J Bacteriol. 2001;183(19):5675-83.
[6] Luli GW, Strohl WR. Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl Environ Microbiol. 1990;56(4):1004-11.
[7] van de Walle M, Shiloach J. Proposed mechanism of acetate accumulation in two recombinant Escherichia coli strains during high density fermentation. Biotechnol Bioeng. 1998;57(1):71-8.
[8] Farmer WR, Liao JC. Reduction of aerobic acetate production by Escherichia coli. Appl Environ Microbiol. 1997;63(8):3205-10.
[9] Kagawa N, Cao Q. Osmotic stress induced by carbohydrates enhances expression of foreign proteins in Escherichia coli. Arch Biochem Biophys. 2001;393(2):290-6.
[10] Tseng CP, Yu CC, Lin HH, Chang CY, Kuo JT. Oxygen- and growth rate-dependent regulation of Escherichia coli fumarase (FumA, FumB, and FumC) activity. J Bacteriol. 2001;183(2):461-7.
[11] Eppler T, Boos W. Glycerol-3-phosphate-mediated repression of malT in Escherichia coli does not require metabolism, depends on enzyme IIAGlc and is mediated by cAMP levels. Mol Microbiol. 1999;33(6):1221-31.
[12] Slavchenko IYu. Isolation of clones sensitive to bacteriophage ? from the phage resistant Escherichia coli strain. Biopolym Cell. 2001; 17(2):160-5.
[13] Slavchenko IYu. The expression of human alpha-2b interferon in different strains of Escherichia coli. Biopolym Cell. 2001; 17(6):546-50.
[14] Kravchenko VV, Guileva IP, Shamin VV, Kulichkov VA, Dobrynin VN, Filippov SA, Chuvpilo SA, Korobko VG. Construction of a tandem of artificial genes encoding human leukocyte interferon and its expression as a part of polycistronic template with coupled translation. Bioorg Khim. 1987; 13 (9):1186-93.
[15] Labinskaya AS. Microbiology with the microbiological studies technique. M.: Meditsina, 1972. 480 p.
[16] Slavchenko IYu. Investigation the influence of cultivation conditions on production of human alpha-2b interferon in Escherichia coli. Biopolym Cell. 2002; 18(2):164-70.
[17] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[18] Jishage M, Ishihama A. A stationary phase protein in Escherichia coli with binding activity to the major sigma subunit of RNA polymerase. Proc Natl Acad Sci U S A. 1998;95(9):4953-8.
[19] Tanaka K, Takayanagi Y, Fujita N, Ishihama A, Takahashi H. Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, sigma 38, is a second principal sigma factor of RNA polymerase in stationary-phase Escherichia coli. Proc Natl Acad Sci U S A. 1993;90(8):3511-5.
[20] Loewen PC, Hu B, Strutinsky J, Sparling R. Regulation in the rpoS regulon of Escherichia coli. Can J Microbiol. 1998;44(8):707-17.
[21] Hengge-Aronis R. Back to log phase: sigma S as a global regulator in the osmotic control of gene expression in Escherichia coli. Mol Microbiol. 1996;21(5):887-93.
[22] Leandro P, Lechner MC, Tavares de Almeida I, Konecki D. Glycerol increases the yield and activity of human phenylalanine hydroxylase mutant enzymes produced in a prokaryotic expression system. Mol Genet Metab. 2001;73(2):173-8.
[23] Fang A, Demain AL. Influence of aeration and carbon source on production of microcin B17 by Escherichia coli ZK650. Appl Microbiol Biotechnol. 1997;47(5):547-53.