Biopolym. Cell. 2002; 18(4):307-311.
http://dx.doi.org/10.7124/bc.000610
Structure and Function of Biopolymers
Glutathione S-transferase PI activity and expression in human placenta depending on CYP1A1 genotype and expression level
1Teplyuk N. M., 1Lebedeva L. M., 1Sazonova L. Ya., 1Samoylenko A. A., 1Scherbina M. S., 1Perepelyuk M. M., 1Obolenskaya M. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The cytosollc glutathione S-transferase activity was studied in human placenta samples of different CYP1A1, GSTP1 and GSTM1 genotypes. The GSTPl gene expression was also detected in human choriocarcinoma cells (BeWo) under conditions of the CYP1AI short-time induction by benzo(a)pyrene. There was a positive correlation between GSTP1 activity/expression and CYP1A1 activity/expression in full-term human placenta under physiological conditions as well as upon the short-time CYP1A1 induction in BeWo cells.
Full text: (PDF, in Ukrainian)

References

[1] Teplyuk NM, Lebedeva LM, Kolomiets LI, Butkevich DM, Khoronzhi MR, Puar'e MS, Obolenskaia MIu. Pheno-and genotyping of a placental detoxication system in ecologically unfavorable regions of Ukraine. Ukr Biokhim Zh. 2001;73(3):126-34.
[2] Obolens'ka MIu, ChaÄ­kovs'ka TL, Lebedieva LM, Teplyuk NM, Kolomiiets' LI, Ivans'ka NV, Didenko LV, Nekrich VV, Burlak HF. Detoxicating function of the placenta of childbearing women from ecologically unfavorable regions of the Ukraine. Ukr Biokhim Zh. 1998;70(2):89-97.
[3] Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974;249(22):7130-9.
[4] Shields PG, Bowman ED, Harrington AM, Doan VT, Weston A. Polycyclic aromatic hydrocarbon-DNA adducts in human lung and cancer susceptibility genes. Cancer Res. 1993;53(15):3486-92.
[5] Harries LW, Stubbins MJ, Forman D, Howard GC, Wolf CR. Identification of genetic polymorphisms at the glutathione S-transferase Pi locus and association with susceptibility to bladder, testicular and prostate cancer. Carcinogenesis. 1997;18(4):641-4.
[6] Comstock KE, Sanderson BJ, Claflin G, Henner WD. GST1 gene deletion determined by polymerase chain reaction. Nucleic Acids Res. 1990;18(12):3670.
[7] Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156-9.
[8] Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Lab. press, 1989. 545 p.
[9] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[10] Zimniak P, Nanduri B, Piku?a S, Bandorowicz-Piku?a J, Singhal SS, Srivastava SK, Awasthi S, Awasthi YC. Naturally occurring human glutathione S-transferase GSTP1-1 isoforms with isoleucine and valine in position 104 differ in enzymic properties. Eur J Biochem. 1994;224(3):893-9.
[11] Kawajiri K, Nakachi K, Imai K, Watanabe J, Hayashi S. The CYP1A1 gene and cancer susceptibility. Crit Rev Oncol Hematol. 1993;14(1):77-87.
[12] Landi MT, Bertazzi PA, Clark G, Lucier GW, Garte SJ, Cosma G, et al. Susceptibility markers in normal subjects: A pilot study for the investigation of 2,3,7,8-tetrachlorodibenzo-p-dioxin related diseases. Chemosphere. 1993;27(1-3):375–81.
[13] Crofts F, Taioli E, Trachman J, Cosma GN, Currie D, Toniolo P, Garte SJ. Functional significance of different human CYP1A1 genotypes. Carcinogenesis. 1994;15(12):2961-3.
[14] Prochaska HJ, Talalay P. Regulatory mechanisms of monofunctional and bifunctional anticarcinogenic enzyme inducers in murine liver. Cancer Res. 1988;48(17):4776-82.
[15] Friling RS, Bensimon A, Tichauer Y, Daniel V. Xenobiotic-inducible expression of murine glutathione S-transferase Ya subunit gene is controlled by an electrophile-responsive element. Proc Natl Acad Sci U S A. 1990;87(16):6258-62.
[16] De Long MJ, Santamaria AB, Talalay P. Role of cytochrome P1-450 in the induction of NAD(P)H:quinone reductase in a murine hepatoma cell line and its mutants. Carcinogenesis. 1987;8(10):1549-53.
[17] Zhang L, Connor EE, Chegini N, Shiverick KT. Modulation by benzo[a]pyrene of epidermal growth factor receptors, cell proliferation, and secretion of human chorionic gonadotropin in human placental cell lines. Biochem Pharmacol. 1995;50(8):1171-80.
[18] Ricci G, Del Boccio G, Pennelli A, Lo Bello M, Petruzzelli R, Caccuri AM, Barra D, Federici G. Redox forms of human placenta glutathione transferase. J Biol Chem. 1991;266(32):21409-15.
[19] Manchester DK, Jacoby EH. Glutathione S-transferase activities in placentas from smoking and non-smoking women. Xenobiotica. 1982;12(9):543-7.
[20] Cervell? I, Giralt M, Nogues MR, Ortin F, Puerto AM, Mallol J. Modifications of glutathione S-transferase (GST) activity in the last period of pregnancy in rats treated with benzo(a)pyrene (BP). Placenta. 1994;15(4):431-40.