Biopolym. Cell. 2000; 16(6):562-572.
http://dx.doi.org/10.7124/bc.000598
Methods
The interaction of cyanine dyes with nucleic acids. 12. Novel monomethyne cyanines based on the 5,6-methylenedioxy-benzothiazole and spectral-luminescent properties of thier complexes with nucleic acids
1Lukashov S. S., 1Losytskii M. Yu., 1Yarmoluk S. M., 2Slominskii Y. L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of organic chemistry NAS of Ukraine
    5, Murmanska Str., Kyiv, Ukraine, 02660

Abstract

Seven novel monomethyne cyanine dyes with the 5,6-methylene-dioxy-benzothiazole ring fused with the heterocycles of different geometry and basisity were synthesised. Their optical properties in presence of dsDNA, UNA and protein were studied. Some of synthesised dyes are perspective objects for further investigations and applying in the NA analysis. The comparison of fluorescent properties of studied dyes and their analogues with unsubstituted benzothiazole ring in presence of nucleic acids allowed us to confirm the hypothesis of «half-intercalation» of monomethyne cyanines into dsDNA.
Full text: (PDF, in Ukrainian)

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