Biopolym. Cell. 2000; 16(3):225-228.
http://dx.doi.org/10.7124/bc.00056A
Structure and Function of Biopolymers
Effects of interferon-α/β on Ca2+ in flux and binding in murine thymocytes
1Dolgaya E. V., 1Rozhmanova O. M., 1Stelmakh L. N., 1Miransky A. V., 2Kudryavets Yu. J.
  1. Bogomoletz Institute of Physiology, NAS of Ukraine
    4, Bogomolets Str., Kyiv, Ukraine, 01024
  2. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022

Abstract

In order to determine the role of calcium in interferon (IFN)-induced immunomodulation, we investigated calcium transport and binding induced by murine IFN-α/β in murine thymocytes. By radiometric method, it was found that a rapid, more than 5-fold, increase in 45Ca2+ influx developed and reached a plateau within 10 min. At the same time the amount of 45Ca2+ associated with the cell surface half decreased. During next 30 min the recovery of 45Ca2+ binding to control level in INF-induced lymphocytes occured. 45Ca2+ influx in thymocytes was dose-dependent. The maximal increase in calcium influx was observed at the IFN concentration of 600 W/ml. Pretreatment of thymocytes with a calcium channel blacker, verapamil, at the doses about 30mkM for 20 min before IFN application significantly decreased 45Ca2+ influx. Depolarization of thymocytes up to 25 mM by increasing the extracellular K+ concentration resulted in complete inhibition of 45Ca2+ influx. Our data indicate that in murine thymocytes IFN-induced calcium influx occcured via voltage-operated calcium channels.
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