Study of tertiary structure elements of tRNASer from Thermus thermophilus in solution

Kovalenko, O. P.; Kriklivyi, I. A.; Tukalo, M. A.

doi:10.7124/bc.00055E

Biopolym. Cell. 2000; 16(2):115-123.

http://dx.doi.org/10.7124/bc.00055E

Structure and Function of Biopolymers

Study of tertiary structure elements of tRNA^Ser from Thermus thermophilus in solution

1Kovalenko O. P., 1Kriklivyi I. A., 1Tukalo M. A.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Reactivity of phosphates and bases in tRNA^Ser T. thermophilus has been studied by chemical modification methods. Ethylnitrosourea has been used to modify phosphate groups, guanosines and cytidines have been modified by dimethylsulphate, adenosines – by diethylpyrocarbonate. The same tertiary interactions exist in the solution structure of tRNASer T. thermophilus as in the crystal one.

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