Biopolym. Cell. 1999; 15(6):529-537.
http://dx.doi.org/10.7124/bc.00054A
Genome and Its Regulation
The analysis of chum salmon insulin-like growth factor I gene sequence: transposones
1Grebenjuk V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Most vertebrates genome contains multiple copies of Tcl-like transposones. Waste majority of them localized in non-expressed part of the genome. Two Tcl-like transposones, designated Tok1 and Tok2 are found in the same orientation in the second and third introns of chum salmon IGF-1 gene. Phylogenetic analysis placed them to salmon SALT(Tss2) and zebrafish Tdr2(Tzf) cluster. The transposase ORFs contain multiple frameshifts and stop codons and have the same orientation in both transposones, but opposite to the ICF-I gene flow. Existence of Tok2 in third introns of both nonallelic salmon IGF-I genes and absence of similar transposones in IGF-l genes of other fish species (perch, zunder, pike, tilapia) apparently shows that they appeared in fish IGF-1 gene after the salmonid branching from common ancestor, but before the tetra-ploidization event. Position of mobile elements in IGF-I gene and the existence in salmon genome expressed sequences, framed by the same or homologous elements, makes possible to suspect the transposition of the exones or genes by the cassette of two transposones. Such transposition, well known in invertebrates, may also exists in vertebrates.
Full text: (PDF, in Russian)

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