Biopolym. Cell. 1999; 15(4):328-336.
http://dx.doi.org/10.7124/bc.00052F
Methods
Interaction of cyanine dyes with nucleic acids. 8. Investigation of cyanine dyes as fluorescent probes for the nucleic acids detection
1Yarmoluk S. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The nineteen polymethyne cyanine dyes with various heterocycles were investigated as possible fluorescent probes for the homogeneous nucleic acid detection in solution. Absorption and fluorescent properties of these dyes, their complexes with DNA and RNA and in the presence of protein were studied. A possible model of "viscous environment" for intensity enhancement of cyanine dyes in their fluorescent complexes with nucleic acids was proposed.
Full text: (PDF, in Ukrainian)

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