Biopolym. Cell. 1999; 15(3):247-251.
Methods
Interaction of cyanine dyes with nucleic acids. 6, Synthesis and spectroscopic properties of thiazole orange–amino acids conjugates
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
The synthesis of amino acid (L-Trp and L-Tyr) derivatives of thiazole orange, monomethirte cyanine dye which significantly increases fluorescence intensity when bound to nucleic acids is described. Hydroxysiiccinimsde ester of Cyan 6 (2-[4-N-Carboxyethyl-quinoline)-methyl]-3-niethylberizihiazole-l,4-p-toluenesulfonatt) was used for the conjugation of dye with amino acids, interaction of obtained conjugates with nucleic acids was investigated using spectroscopic methods.
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