Biopolym. Cell. 1998; 14(2):163-172.
http://dx.doi.org/10.7124/bc.0004CA
Methods
Synthesis and use of disulfide-based H-phosphonate reagent for 3'- and/or 5'-oligonucleotide labelling via mercaptoalkyl linker
1Dubey I. Y., 1Fedoryak D. M.
  1. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

Synthesis of new disulfide-based H-phosphonate reagent for solid-phase oligonucleotide functionalization with mercaptopropyl group at one or two termini is described. Using this single reagent, 3'- and 3', 5'-disulfide-linked oligonucleotides were synthesized', disulfide bonds cleaved with dithiothreitol and generated thiol groups labelled with iodoacetamidofluorescein. Tis oligonucleotides containing one or two fluorescein residues at 3'- and 5'-ends were prepared in high yields.
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References

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