Biopolym. Cell. 1998; 14(1):82-86.
http://dx.doi.org/10.7124/bc.0004BD
Methods
Interaction of cyanine dyes with nucleic acids. 4. Efficient 5'-fluorescent labelling of oligonucleotides with monomethyne pyrylium cyanine dye, Cyan 39
1Yarmoluk S. M., 1Kostenko A. M., 1Kornushyna O. S., 2Dubey I. Y.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

A new system for fluorescent 5'-end labelling of oligonucleotides with cyanine dyes is proposed. Reaction of primary amine with pyrylium salt was used for the efficient conjugation of monomethyne pyrylium cyanine dye with 5'-aminoalkyl oligonucleotide with simultaneous conversion of the «brightless» pyrylium dye Cyan 39 into «bright» pyridinium one Cyan 40.
Full text: (PDF, in English)

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